Carcinogenesis, Teratogenesis & Mutagenesis ›› 1998, Vol. 10 ›› Issue (5): 267-271.doi: 10.3969/j.issn.1004-616x.1998.05.001

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STUDY ON THE ANEUPLOIDY FREQUENCY IN HUMAN SPERM CHROMOSOME USING FLUORESCENCE IN SITU HYBRIDIZATION ( FISH)

Zheng Lukang , Deng Lixia , Zhang qiao   

  1. L ab. of Genetic Toxicology , School of Public Heal th , S un Yat2Sen University of Medical Sciences , Guangz hou 510089
  • Received:1900-01-01 Revised:1900-01-01 Online:1998-09-30 Published:1998-09-30

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Abstract: The f requency of 　Human sperm chromosome aneuploidy was assessed from 10 normal and health adult s by using the method of FISH. Sperm heads were decondensed with dithilthreitol (DTT) and lithium diiodosalicylate (LIS) , then the sperm nuclei were hybridized with biotin labeled alpha satellite X chromosome specific DNA probe (DXZ1) in 37 ℃ overnight . The hybridization signals were detected with Cy32St reptavidin. The red hybridization signals in X sperms were clearly visible under the Nikon fluorescence microscope. The hybridization efficiency was 48. 62 % ( rance 47. 54 % to 49. 36 %) . A total 55453 X sperm nuclei were observed. The frequency of disomic sperms was 0. 06 %. There was no significant difference observed in disomic frequency of X sperms between ours and that reported by former authors. The information provided by this paper is valuable for further studies on men exposed occupationally or environmentally to potential aneuploidogenic agents.

Key words: Fluorescence in situ hybridization (FISH), Human sperm chromosome, Aneuploidy frequency